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2018 Vol.5, Issue 3 Preview Page

Original Article

Amended Soil with Biopolymer Positively Affects the Growth of Camelina sativa L. Under Drought Stress

가뭄 조건 하에서 바이오폴리머 혼합 토양이 Camelina sativa L.의 생장에 미치는 긍정적 영향

Hyun-Gyu Lim1
,
Hyun-Sung Kim2
,
Hyeon-Sook Lee1
,
Jung-Ho Sin1
,
Eun-Suk Kim3
,
Hyo-Seop Woo3
,
Sung-Ju Ahn4*
임 현규1
,
김 현성2
,
이 현숙1
,
신 정호1
,
김 은석3
,
우 효섭3
,
안 성주4*
1Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Korea
2Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Korea
3Department of Earth Science and Environmental Engineering, Gwangju Institute of Science and Technology School, Gwangju 61005, Korea
4Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Korea
1전남대학교 바이오에너지공학과 석사 과정
2전남대학교 바이오에너지공학과 박사 과정
3광주과학기술원 지구･환경공학부 교수
4전남대학교 바이오에너지공학과 교수
* Corresponding Author
30 September 2018. pp. 163-173
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Abstract

The biopolymer (BP) used in this study is mainly composed of xanthan gum and β-glucan derived from microorganism and has been introduced as a novel material for soil stabilization. However, the broad applicability of BP has been suggested in the field of geotechnical engineering while little information is available about the effects of BP on the vegetation. The goal of this study is to find the BP effects on the growth of Camelina sativa L. (Camelina) under drought condition. For more thorough evaluation of BP effects on the plant growth, we examined not only morphological but also physiological traits and gene expression patterns. After 25 days of drought treatment from germination in the soil amended with 0, 0.25, 0.5, and 1% BP, we observed that the BP concentration was strongly correlated the growth of Camelina. When plants were grown under drought stress, Camelina in 0.5% BP mixture showed better physiological parameters of the leaf stomatal conductance, electrolyte leakage and relative water content compared to those in control soil without BP. Plant recovery rate after re-watering was higher and the development of lateral root was lower in BP amended soil. RNA expression of Camelina leaf treated with/without drought for 7 and 10 days showed that aquaporin genes transporting solutes at bio-membrane, CsPIP1;4, 2;1, 2;6 and TIP1;2, 2;1, were induced more in the plants with BP amendment and drought treatment. These results suggest that the soil amended with BP has a positive effect on the transport of nutrients and waters into Camelina by improving water retention in soil under drought condition.

Keywords
Aquaporin
Biopolymer
Camelina sativa L.
Water retention

본 연구에서 사용된 바이오폴리머는 미생물로부터 추출한 β-glucan 및 xanthan gum 이 주성분인 친환경 신소재이며, 토양에 처리하면 강도 증진을 통한 제방의 침식 및 유실 감소뿐만 아니라 토양 내 수분보유력을 증가시키는 것으로 보고되어 있다. 그러나 바이오폴리머가 제방에 적용되었을 때 주변 식생에 미치는 영향에 대한 연구는 미흡한 상황이다. 본 연구는 가뭄 조건에서 토양 내 바이오폴리머 혼합 유무에 따라 Camelina sativa L. (Camelina)의 생육에 미치는 영향을 알아보고자 하였다. 각각 0, 0.25, 0.5, 1%의 바이오폴리머가 혼합된 토양에서 발아로부터 25일간 가뭄 처리 후 표현형을 관찰한 결과, 혼합된 바이오폴리머 농도가 높을수록 가뭄 피해가 감소하였다. 특히 25일 동안의 가뭄 처리된 0.5% 바이오폴리머 혼합구에서 신장, 엽장, 엽폭이 대조구 보다 각각 약 30, 70, 170% 증가하였다. 이후 재급수를 통한 바이오폴리머 혼합구의 회복률 또한 약 80% 높게 나타났다. 식물의 근권 발달을 보기 위해 20일 동안 가뭄을 처리하였으며, 대조구에 비해 바이오폴리머 혼합구에서 측근 형성 및 발달이 약 40% 감소한 것을 확인하였다. Camelina에 가뭄 처리하여 기공전도도, 전해질 유출도, 상대적 수분함량 등 생리적 반응을 조사한 결과, 바이오폴리머의 혼합이 가뭄으로 인한 피해를 각각 약 50, 70, 50% 감소시켰다. 바이오폴리머가 가뭄 조건 하에서 식물의 생장에 미치는 영향을 분자수준에서 알아보기 위해 Reverse Transcription- Polymerase Chain Reaction (RT-PCR)을 통한 유전자 발현양상을 분석하였다. 수분수송 관련 유전자 (aquaporin)인 PIP1;4, 2;1, 2;6 및 TIP1;2, 2;1 발현이 바이오폴리머 혼합구 및 가뭄 처리된 Camelina 잎에서 더 높게 나타났다. 이러한 결과를 종합하였을 때, 바이오폴리머는 가뭄 조건에서 토양 내 수분보유력을 유지시킴으로써 Camelina의 양·수분의 흡수 및 생육에 긍정적인 영향을 주는 것으로 보인다.

키워드
Aquaporin
바이오폴리머
Camelina sativa L.
수분보유력
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Information
  • Publisher :Korean Society of Ecology and Infrastructure Engineering
  • Publisher(Ko) :응용생태공학회
  • Journal Title :Ecology and Resilient Infrastructure
  • Journal Title(Ko) :응용생태공학회 논문집
  • Volume : 5
  • No :3
  • Pages :163-173
  • Received Date : 2018-09-07
  • Revised Date : 2018-09-20
  • Accepted Date : 2018-09-21
  • DOI :https://doi.org/10.17820/eri.2018.5.3.163
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